Staff Publications 
The Biotech Advisor
Biotechnology Consulting Services
Martin A. Winkler, Ph.D. 
O R I G I N A L    A R T I C L E S

1. Winkler, M.A. and Sanders, B.G.  Chemical and immunologic characterization of a beta-2-microglobulin-like protein isolated from chicken sera.  Immunochemistry 14:615-619, (1977).

2. Winkler, M.A., Merat, D., Tallant, E.A., Hawkins, S., and Cheung, W.Y. Catalytic site of calmodulin-dependent phosphatase from bovine brain residues in subunit A. Proc. Nat.. Acad. Sci. (USA) 81: 3054-3058 (1984).

3. Winkler, M.A., Zysk, J.R., and Cheung, W.Y. Production and characterization of a monoclonal antibody cross-reactive with calmodulin, calmodulin-dependent phophodiesterase, and protein phosphatase. Methods Enzymol. 139:505-518, (1987).

4. Winkler, M.A., Fried, V.A., Merat,.D.L., and Cheung, W.Y.  Differential reactivities of lysines in calmodulin complexed to phosphatase.  J. Biol. Chem. 262:15466-15471 (1987).

5. Anthony, F.A., Winkler, M.A., Edwards, H.H., and Cheung, W.Y. A quantitative subcellular localization of calmodulin-dependent phosphatase in chick forebrain  J.Neuroscience 8:1245-1253 (1988).

6. Pezzi, L., Merat, D.L, , Winkler, M.A., and Cheung, W.Y. Calmodulin-dependent phosphatase preferentially dephosphorylates a 28 kD protein in human platelets.  Int. J. Biochem. 21:791-798 (1989).

7. Bush, D.A. and Winkler, M.A.  Isoelectric focusing of cross-linked monoclonal antibody heterodimers, homodimers, and derivatized monoclonal antibodies.  J.Chromatography (Biomedical Applications) 489:303-311 (1989).

8. Foglesong, D.P., Winkler, M.A., Price, J.O, Reagh, S., Marshall, G., Hixson, K., and West, W.H. Preparation and analysis of bifunctional immunoconjugates containing monoclonal antibodies OKT3 and BABR1. Cancer Immunology and Immunotherapy 30:177-184 (1989).

9. Winkler, M.A., Price, J.O., Foglesong, D.P., and West, W.H.  Biodistribution and plasma survival in mice of anti-melanoma monoclonal antibody cross-linked to OKT3. Cancer Immunology and Immunotherapy 31:278-284 (1990).

10. Winkler, M.A., Brashear, R.J., Schur, J.D., Hall, H.J., and Pan, A.A. Detection of antibodies to Trypanosoma cruzi among blood donors in the Southwestern and Western United States. II. Evaluation of a supplemental enzyme immunoassay and radioimmunoprecipitation assay for confirmation of seroreactivity. Transfusion 35: 219-225 (1995).

11. Brashear, R.J., Winkler, M.A., Schur, J.D., Lee, H., Burczak, J., Hall, H.J., and Pan, A.A. Detection of antibodies to Trypanosoma cruzi among blood donors in the Southwestern United States.  I. Evaluation of the sensitivity and specificity of an enzyme immunoassay for detecting antibodies to T. cruzi.  Transfusion 35:213-218 (1995).

12. Winkler, M.A., Kundu, S., Robey, T.E., and Robey, W.G. Comparative Peptide Mapping of a Hepatitis C viral recombinant protein by capillary electrophoresis and MALDI-TOF Mass Spectrometry.  Journal of Chromatography , 744:177-185 (1996) 

13.  Kundu, S., Fenters, C., Lopez, M., Calfin, B., Winkler, M., and Robey, W.G.  Purity testing of  recombinant proteins by capillary electrophoresis.  Journal of Capillary Electrophoresis, 3:301-307 (1997).

14. Winkler, M.A., Rivera, D.M., Pan, A.A., and Nowlan, S.F.  Homology of Trypanosoma cruzi clone        36 repetitive DNA sequence to sequence encoding Human RO/SSA 52KD Autoantigen.  Parasite. 5: 94-95 1998)

15.   Winkler, M.A., Uher, J., and Cepa, S.   Direct Analysis and Identification of Helicobacter and 
Campylobacter species by MALDI-TOF Mass Spectrometry.  Analytical Chemistry 71:3416-3419 (1999). 

16. Basombrio, M.A., Segovia, A., Ramos, M.P., Esteban, E., Strumpf, R., Jurgenson, P., Winkler, M.A., Sayre, K., and Ferrer, J.F.  Endemic Trypanosoma Cruzi infection in Indian populations of the Gran Chaco territory of South America: performance of diagnostic assays and epidemiological features.  Annals of Tropical Medicine and Parasitology 93: 41-48 (1999)

17. Winkler, M.A., Xu, N., Wu, H., and Aboleneen, H.  MALDI-TOF MS of Chemically modified Recombinant Hepatitis B Surface Antigens.  Analytical Chemistry 4:664A-667A (1999).

18. Winkler, M.A., Hickman, R.K., Golden, A., and Aboleneen, H.   Analysis of Escherichia Coli 
Recombinant Protein Expression by MALDI-TOF Mass Spectrometry of Bacterial Colonies.  
 Biotechniques 28: 890-895 (2000).

19.   Campbell, R., Winkler, M.A., and Wu, H.  Quantification of sodium dodecyl sulfate in microliter biochemical samples by gas chromatography.  Analytical Biochemistry 335: 98-102 (2004).

20.   Winkler, M.A. and Pan, A.A.  Is there a link between the human TRIM21 and Trypanosoma cruzi Clone 36 genes in Chagas’ disease?  Molecular Immunology 48:365-367 (2010).


B O O K   C H A P T E R S   A N D   R E V I E W S

1. Winkler, M.A., DeWitt, L., and Cheung, W.Y.  Calmodulin and calcium channel blockers. Hypertension 9:217-223 (1987).

2. Marshall, G.D., Foglesong, P.D., Price, J.O., Winkler, M.A., and West, W.H. Increased antigen-specific lytic activity of interleukin-2 activated cells by heteroconjugate antibodies.  Immunology and Allergy Clinics of North America 8:126-128 (1988).

3. Pan, A.A. and Winkler, M.A.  The Threat of Chagas’ Disease in Transfusion Medicine. Laboratory Medicine, 28: 269-274 (1997).

P A T E N T S

1.     Winkler, M.A and Pan, A.A.   Assay for Trypanosoma cruzi antibodies which specifically bind three different antigens.  U.S. Patent No. 5,550,027.  Issued 8/27/96.

2.     Winkler, M.A. and Pan, A.A.   Process for Purifying the Gp 60/50 Antigen of  T. cruzi.  U.S. Patent No.  5,583,204 issued 12/10/96.

3.     Winkler, M.A. and Pan, A.A.   Process for linking an antigenic glycolipid of T. cruzi to a protein carrier.  U.S. Patent No. 5,623,058 issued 4/22/97.

4.     Winkler, M.A.  and Pan, A.A.    Assay for Chagas’ Disease and reagents for its use.  U.S. Patent No. 5,645,838 issued 7/08/97.


A B S T R A C T S

1.Winkler, M.A., Prager, D., and Sanders, B.G. Chicken beta-2 -microglobulin is a possible chemotactic factor for chicken peripheral blood monocytes. Fed. Proc. 37:1564, (1978).

2.Winkler, M.A., and Sanders, B.G. Avian homologue of beta-2 -microglobulin isolated from chicken sera. Fed. Proc. 36:1199, (1977).

3.Winkler, M.A., and Sanders, B.G. Beta-2-microglobulin in chickens: Isolation and characterization. Genetics 83:s83, (1976).

4.Winkler, M.A., Zysk, J.R., and Cheung, W.Y. Monoclonal antibody which recognizes calmodulin and calmodulin-dependent phosphodiesterase and protein phosphatase. Fed. Proc. 44:1776,(1985).

5.Anthony, F.A., Winkler, M.A., Edwards, H.H., and Cheung, W.Y. Quantitative subcellular localization of calmodulin-dependent phosphatase in chick forebrain. Fed. Proc. 46:2077, (1987).

6.Winkler, M.A., Foglesong, P.D., Price, J.O., West, W.H., and Marshall, G.D. Biochemical and biological stability of anti-CD3 monoclonal antibodies cross-linked to antitumor antibodies. FASEB J. 2:A692 , (1988).

7.Pan, A.A., Brashear, R.J., Winkler, M.A., and Lee, H.  A specific and sensitive enzyme immunoassay and confirmatory test for the detection of antibody to Trypanosoma cruzi (Chagas' Disease). Transfusion 31:15s (1991)

8.Pan, A.A., Brashear, R.J., Winkler, M.A., and Lee, H. An enzyme immunoassay and confirmatory test for the detection of antibody to T. Cruzi. Amer. J. Trop. Med. Hyg. 45:120 (1991).

9.Pan, A.A., Brashear, R.J., Schur, J..D., Winkler, M.A.,Hall,H., Shih, J., and Decker, R. Prevalence study and confirmation of seropositive antibodies to Trypanosoma cruzi (Chagas' disease) in hispanic and non-hispanic blood donors in the United States. Saulo Paulo Med. J. 110 (5): 18 (1992).

10.Pan, A.A., Brashear, R.J., Schur, J.D., Winkler, M.A., Shih, Decker, R. Chagas' Disease among blood donors in North and South America. The prevalence of seroreactive antibodies to T Trypanosoma cruzi in the United States and Argentina. Transfusion 32: 65s (1992).

11.Brashear, R.J., Winkler, M.A., Schur, J.D., Hall, H.J., Burczak, J.D., and Pan, A.A. Chagasic Antibody positive blood donor prevalence in the U.S. hispanic surname populations. Clinical Chemistrv 39:1246 Abstract 6 (1993).

12.Pan, A.A., Schur, J.D., Brashear, R.J., Winkler, M.A.,Cantrell L., Rivera, D., Shih, J., and Holzer, T. Evaluation of the Chagas Antibody Enzyme Immunoassay for a blood-borne pathogen. Transfusion 33(9S) :43S Abstract S160 (1993).

13.Pan, A.A., Schur, J.D., Brashear, R.J., Winkler, M.A.,Cantrell L., Rivera, D., Shih, J., and Holzer, T. High seroprevalence of Chagas antibody positive specimens found in the U.S. Should a diagnostic test for Trvpanosoma cruzi be implemented? American Journal of Tropical Medicine and Hvgiene 49(3) :380-381 (1993).

14.Winkler, M. A., Robey, T.E., Kundu, S., and Robey, W.G.  Comparative Peptide Mapping of a Hepatitis C Viral Recombinant Protein by Capillary Electrophoresis and MALDI-TOF Mass Spectrometry. 8th International Symposium on High Performance Capillary Electrophoresis January 21, 1996.

15.Allen, S., Turowski, E., Thomas, R., Steckel, El, O’Hara, R., Pysto, W., Winkler, M., Hoff-Velk, J., Schmidt, D., Junge, D., Payete, D., Lundquist, E., Peiczynski, W., Zeck, B., Schmidtke, H., Hoffman, T., and Shumard-Sauer, C.  Pluronic F68 Binds Non-Specifically to Mouse Monoclonal Antibodies Produced in Hybridoma Serum-Free Media.  Proc. Amer. Soc. Microbiol. Annual Meeting,  May, 1999.

16.Wu, H., Winkler, M., and Aboleneen, H.  Differentiation of Compounds Containing Polyoxyethylene Units by Tandem Mass Spectrometry. Proceedings of the  46th ASMS Conference on Mass Spectrometry, May 1998.

17.Xu, N., Wu, H., Winker, M., and Aboleneen, H.  Mass Spectrometric Analysis of Human Cardiac Troponin I Used in Immunoassay.   Proceedings of the  49th ASMS Conference on Mass Spectrometry, May 27-31, 2001.